Composite

Part:BBa_K590064:Design

Designed by: Matthew Harger   Group: iGEM11_Washington   (2011-10-25)


The FabBrick: FabH2, an enzyme for changing up fatty acid biosynthesis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
    Illegal AgeI site found at 1954
    Illegal AgeI site found at 2593
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1999


Design Notes

Needed to dramatically lower expression of FabH2.


Source

Synthesized from the Bacillus subtilis protein sequence

References